Alexandre de Brevern - Thèse de Bioinformatique Moléculaire

abstract

The "Hybrid Protein Model" (HPM) is a fuzzy model for compacting local protein structures. It learns a non-redundant database encoded in a previously defined structural alphabet composed of 16 protein blocks (PBs) [35]. The hybrid protein is composed of a series of distributions of the probability of observing the PBs. The training is an iterative unsupervised process that for every fold to be learnt consists of looking for the most similar pattern present in the hybrid protein and modifying it slightly. Finally each position of the hybrid protein corresponds to a set of similar local structures. Superimposing those local structures yields an average root mean square of 3.14 Å. The significant amino acid characteristics related to the local structures are determined. The use of this model is illustrated by finding the most similar folds between two cytochromes P450.

Camproux AC, de Brevern AG, Tufféry P, et Hazout S, "Exploring the use of a structural alphabet for a structural prediction of protein loops", Theoretical Chemistry Accounts, sous presse [21].